How to use (3 steps)
- Select nucleic acid type (dsDNA/RNA, etc.) and enter A260/A280/A230.
- If measured with dilution, enter the dilution factor (no dilution = 1).
- Concentration and ratios appear automatically. Add volume to see total amount.
A260 factors and ratio guides vary by sample and conditions. They are shown as references; confirm with your assay.
Input (type, A260/A280/A230, dilution)
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dsDNA=50 and RNA=40 are common guides. Use a custom factor if needed.
Blank correction (optional)
Many instruments already apply blank correction. Enable only if needed.
Results (concentration & ratios)
Results will appear here once you enter values.
About ratio guides (reference)
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1 µg/mL = 1 ng/µL (same numeric value).
Ratio interpretation depends on the assay; this page does not make definitive judgments.
Export (copy / CSV / LaTeX / share URL)
How it’s calculated
- Concentration (µg/mL) = A260 × factor × dilution (dsDNA=50, RNA=40 are guides)
- A260/280 = A260 ÷ A280 (A280 ≠ 0)
- A260/230 = A260 ÷ A230 (A230 ≠ 0)
- Entering volume calculates total amount (ng/µg)
FAQ
Is the dsDNA factor of 50 fixed?
It is a commonly used guide, but it can vary by sample and conditions. Use a custom factor if needed.
What do A260/280 and A260/230 indicate?
They are often cited as guides for protein or salt/solvent contamination, but they are not definitive.
What does entering volume show?
It calculates the total amount (ng/µg) in the tube from the concentration.
Do I need blank correction?
Many instruments correct internally. Enable it only if needed.
Is the data sent anywhere?
All calculations run in your browser; data is not sent.
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