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- DNA assembly mix calculator (Gibson/Golden Gate) | Auto mix volumes | CalcBE Calculate DNA assembly mix for Gibson or Golden Gate. From backbone/insert molar ratios, get required mass (ng) and volume (µL), plus remaining wat...
- NGS library concentration (ng/µL → nM) | dilution & pooling | CalcBE Convert NGS library concentration from ng/µL and fragment length to nM, then calculate normalization dilutions and pooling (equal volume or equal m...
- PCR master mix calculator | reactions, concentrations, overage | CalcBE Calculate PCR/qPCR master mix volumes from reaction volume/count and stock/final concentrations. Supports overage (% or +1), 96/384 wells, protocol...
- Primer Tm calculator | PCR annealing temperature guide | CalcBE Calculate primer Tm from DNA sequences (5'→3') and estimate annealing temperature (Ta). Switch between recommended nearest-neighbor and simple form...
- Ligation molar ratio calculator | Vector:insert amounts | CalcBE Calculate required insert mass (ng) and volume (µL) from vector mass, length, and desired molar ratio (e.g., 1:3). Includes presets, copy, CSV/JSON...
- Cell seeding calculator | cells/well → required volume | CalcBE Calculate required cell suspension and media volume from target cells/well (or cells/cm²), number of wells, volume, and stock concentration. Includ...
- Growth curve fitter (exponential & logistic) | doubling time & K | CalcBE Fit exponential and logistic growth to time-series data and estimate growth rate r, doubling time, and carrying capacity K. View fitted curves, res...
- ΔCt / ΔΔCt calculator (relative expression) | CalcBE Paste qPCR Ct values to calculate ΔCt/ΔΔCt and relative expression (2^-ΔΔCt). Supports calibrator selection, multiple reference genes, Pfaffl effic...
- qPCR standard curve calculator (efficiency, R², unknowns) | CalcBE Build a qPCR standard curve from Quantity and Ct, calculate slope, intercept, R², and PCR efficiency (%). Back-calculate unknown quantities with re...
- Dirichlet Distribution Generator & Visualizer (probability vectors) | CalcBE Generate probability vectors from a Dirichlet distribution and visualize marginals and simplex plots. Runs locally — no uploads; share settings-onl...
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- Concrete, Gravel & Mulch Calculator | Material Estimates | CalcBE
Estimate concrete volume and bag counts, gravel coverage and tons, and mulch bags with US or metric inputs plus a waste allowance. Results are informational only.
- Function Transformations Explorer (Translate, Scale, Reflect) | CalcBE
Adjust a, b, h and v in y = a·f(b(x−h)) + v to explore shifts, stretches and reflections. Compare graphs side by side. Shareable URL. No sign-in.
- Long Division Calculator (Steps, Quotient, Remainder) | CalcBE
See quotient, remainder, and step-by-step long division with layout. Supports decimals and repeating decimals. Teacher mode, shareable URL, LaTeX. No sign-in.