How to use (3 steps)
- Choose an example or select the chamber (default: improved Neubauer) and counting scheme.
- Enter dilution factor and square counts (Live/Dead or Total).
- Click “Calculate” to see cells/mL, viability, and variability guide (auto updates if enabled).
Inputs (chamber, dilution, counts)
Results (cells/mL & viability)
cells/mL (total)
—
cells/mL (live)
—
cells/mL (dead)
—
Viability (%)
—
Dilution factor
—
Squares counted
—
Volume per square (mL)
—
Variability guide
| mean | SD | CV | |
|---|---|---|---|
| Live | — | — | — |
| Dead | — | — | — |
| Total | — | — | — |
Counts by square (bar chart)
How it’s calculated
- Volume per square (mL) = area (mm²) × depth (mm) × 10−3
- cells/mL = mean count ÷ (volume per square in mL) × dilution factor
- Viability (%) = Live ÷ (Live + Dead) × 100 (Live/Dead mode)
Warnings are guides, not definitive. Adjust dilution or number of squares to fit your workflow.
FAQ
Why multiply by 10^4?
In an improved Neubauer chamber, the large square volume is 1e-4 mL, so the conversion factor is 10^4 (check the settings).
It says counts are low/high.
Low counts increase error and high counts are hard to count. Adjust dilution or the number of squares as needed.
How is viability calculated?
If you count live and dead separately, viability = Live/(Live+Dead)×100.
Does the share URL include counts?
Yes. If the URL gets too long, you can switch to sharing without data.
Is my data sent anywhere?
No. Calculations run in your browser and data is not sent.
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